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. 2018 Nov 20;13:7727–7747. doi: 10.2147/IJN.S182384

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© 2018 Naseri et al. This work is published and licensed by Dove Medical Press Limited

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Characterization of exosomal particles.

Notes: (A) Transmission electron micrograph of negatively stained exosomes with a diameter of 30–150 nm, labeled for CD63 (Low magnification, 13,000×, scale bar =150 nm, and high magnification, 40,000×, scale bar =60 nm). (B) Western blot analysis of positive and negative exosomal CD markers in both isolated exosomes and cellular lysate: (1) CD63 expression in exosomes lysate, (2) CD81 expression in exosomes lysate, (3) protein marker, (4) non-expression of calnexin in exosomes lysate, (5) the existence of calnexin in the cell lysate. (C) Size distribution by intensity (i) and size distribution by number of MSCs-Exo (ii).

Abbreviations: CD, cluster of differentiation; MSCs-Exo, mesenchymal stem cells-derived exosomes; Pdi, polydispersity index.