Figure 6.

The effect of the LNA-anti-miR-142-3p loaded exosomes on cell apoptosis.

Notes: 4T1 and TUBO cells were co-cultured with different MSCs-derived exosome preparations for 48 hours followed by flow cytometry analysis of apoptotic cells. (A) (i) Representative dot plots of TUBO cancer cells, (ii) percentage of early apoptosis and late apoptosis in TUBO cells (*P<0.05). (B) (i) Representative dot plots of 4T1 cancer cells, (ii) percentage of early apoptosis (positive to Annexin V and negative to PI) and late apoptosis (positive to Annexin V and PI) in 4T1 cells treated with LNA-anti-miR-142-3p loaded exosomes showed significant enhancement vs untreated cells or treated cells with unloaded exosomes and anti-miR negative control loaded exosomes (*P<0.05). The data represent three independent experiments and are expressed as mean ± SEM.

Abbreviations: Exo + anti-miR-142-3p, exosomes loaded with LNA-anti-miR-142-3p; Exo + anti-miRNC, exosomes loaded with LNA-anti-miRnegative control; MSCs, mesenchymal stem cells; LNA, locked nucleic acid; SEM, standard error of the mean.