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. Author manuscript; available in PMC: 2019 Nov 21.
Published in final edited form as: Neuroscience. 2018 Nov 21;393:369–380. doi: 10.1016/j.neuroscience.2018.09.033

Figure 5. PNHFD decreased the number of NOS-IR, but not ChAT-IR neurons in myenteric ganglia.

Figure 5.

A Number of cholinergic (ChAT-IR) myenteric neurons in the fundus (left), corpus (middle), and duodenum (right) of control and PNHFD rats. Note that while there was a trend towards a decrease in number of cholinergic neurons, this did not reach significance during the time-period of the current study (N=3 per data point)

B. Number of nitrergic (NOS-IR) myenteric neurons in the fundus (left), corpus (middle), and duodenum (right) of control and PNHFD rats. A significant decrease in number of nitrergic neurons was observed in all regions studied, from 4 weeks of age in the fundus, and by 6 weeks of age in the copurs and duodenum. The loss of NOS-IR neurons remained consistent throughout the duration of the study. (N=3 per data point; *P<0.05; one way ANOVA followed by post hoc Bonferroni comparison between groups)

C. Representative images of ChAT-IR (red; left) and merged ChAT-IR and PGP9.5-IR (green; right) in control (upper) and PNHFD (lower) corpus myenteric ganglia of 12 week old rats. Scale bar = 50μm.

D: Representative images of NOS-IR (red; left) and merged NOS-IR and PGP9.5-IR (green; right) in control (upper) and PNHFD (lower) corpus myenteric ganglia of 12 week old rats. Note the decrease in number of NOS-IR following PNHFD. Scale bar = 50μm.