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. 2017 Aug 31;76(10):898–907. doi: 10.1093/jnen/nlx074

FIGURE 6.

FIGURE 6

αEnsa protein is upregulated and nuclear translocated in ischemic rat neurons. Immunoperoxidase preparations reveal αEnsa protein upregulation in ischemic cortical neurons, relative to control (A, upper row). Double label immunofluorescent images demonstrate colocalization with NeuN in ischemic neurons with nuclear translocation seen in zones 1 and 2 (A, rows 2–4, with yellow merged images in lower row). Nuclear translocation of αEnsa is further demonstrated by DAPI colabeling in zones 1 and 2 (B). Quantitative ROI analysis of nuclear labeling is illustrated (C); scale bars, 10 µm (A) or 20 µm (B); αEnsa, red/CY3; NeuN, green/FITC; nuclei, blue/DAPI.