Skip to main content
. 2017 Feb 8;26(7):1305–1317. doi: 10.1093/hmg/ddx046

Figure 6.

Figure 6

SK2 is hyperphosphorylated in an HD mouse model. (A) The cortex and striatum were obtained from wild-type (WT) and BACHD (HD) mouse brains, homogenized and processed by immunoblotting with antibodies against mHtt. Actin was used as a loading control. (B) Cortices and striata were dissected from wild-type (WT) and BACHD (HD) mouse brains, homogenized, and processed by immunoblotting with antibodies against phosphorylated histone H2A.X, γH2A.X. Actin was used as a loading control. (C) Levels of γH2A.X were normalized to actin in WT and BACHD brain samples. *P = 0.018 and *P = 0.046 (t-test) for cortical and striatal neurons, respectively. Results were pooled from three WT and three BACHD mice. (D) The cortex and striatum were obtained from wild-type (WT) and BACHD (HD) mouse brains, homogenized and processed by immunoblotting with antibodies against pan-SK2 (SK2) and phosphorylated SK2 (pSK2). Actin was used as a loading control. (E) Levels of pan SK2 (pan) and phosphorylated SK2 (phospho) were normalized to actin in WT and BACHD brain samples. *P = 0.046 (t-test). n.s., not significant (P = 0.15). Results were pooled from three WT and three BACHD mice. (F) Cortical wild-type (WT) and BACHD (HD) neurons were transfected with mApple, fixed, and immunostained with an antibody against phosphorylated SK2 (pSK2), and with the nuclear Hoechst dye (DAPI). Scale bar is 10 μm. White arrows depict transfected neurons. (G) Quantification of the nuclear phosphorylated SK2 fluorescence intensity from (F). ***P < 0.001 (t-test). A.u., arbitrary units. Two hundred neurons were analyzed from two independent experiments.