Figure 2.

Global gene expression changes of C. difficile 630 in iron depleted media. (A) Hierarchical clustering of microarray data. Genes were identified as significant by the J5 test and fold-change across three independent microarray experiments. Statistically significant genes were grouped by hierarchical clustering using gene pattern and clustered using the Pearson correlation. (B) Validation of microarray data. Gene expression changes were measured by quantitative real-time PCR at 7 h from three independent cultures. The results of qPCR are represented with white bars, while corresponding values from the microarray experiments are represented with black bars. Data are presented as mean ± SEM of log2 transformed fold-change where fold change is the ratio of expression in iron depleted BHIS to BHIS alone.