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(A) Representative fluorescence image of cultured murine motor neurons ectopically expressing flag-TRPV4 following intranuclear microinjection of plasmids encoding flag-TRPV4 and EGPF at 20 µg/mL. Scale bar: 20 μm. (B) Viability of motor neurons expressing EGFP alone (control) or together with Flag-TRPV4 at 2 concentrations (20 or 45 µg/mL) over 5 days. Note the strong toxicity in motor neurons overexpressing TRPV4 at day 2. *p < 0.05 versus control *p < 0.05, Student test.
