Figure 1.

Detection of localization of TmeA, IncA and CT005 with the split-GFP system during C. trachomatis infection. (A) The constitutive expression vector was constructed by ligating the chlamydial pSW2 fragment to a modified E. coli vector that contains an origin (ori) and beta-lactamase gene (bla). Constitutive expression of GFP11-fused effector proteins is driven by the chlamydial DnaK promoter. The restriction sites NcoI and NotI were used to insert genes encoding chlamydial effector proteins. (B) Chlamydia trachomatis transformants expressing control vector, IncA-GFP11, CT005-GFP11 or TmeA-GFP11 were used to infect A549-GFP1-10 cells at a multiplicity of infection (MOI) of 10. The localization of IncA, CT005 and TmeA were visualized at 24 hpi by live imaging, respectively. Scale bar, 32 μm.