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GFP1-10 staining assay for chlamydial protein localization. Chlamydia trachomatis strains expressing IncA-GFP11, TmeA-GFP11, CT005-GFP11 or CT733-GFP11 were used to infect Hela cells with an MOI of 10. The cells were fixed at 24 hpi with 4% paraformaldehyde and then permeabilized with 0.1% Triton X-100 as described in Experimental procedures. The localization of IncA (A), CT005 (B), TmeA (C) and CT733 (D) was detected by staining with purified GFP1-10 fragment. Scale bar, 16 μm.
