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. 2018 Nov 23;7:e41497. doi: 10.7554/eLife.41497

Key resources table.

Reagent type
(species) or
resource		 Designation Source or
reference		 Identifiers Additional
information
Strain, strain
background
(N. crassa)		 WT FGSC#6103 N623 mat A; his-3
Strain, strain
background
(N. crassa)		 WT Honda and Selker, 2008 N2930 mat A; his-3;
∆mus-52::bar
Strain, strain
background
(N. crassa)		 WT FGSC#2489 N3752 mat A
Strain, strain
background
(N. crassa)		 WT FGSC#4200 N3753 mat a
Strain,
strain background
(N. crassa)		 ash1-3xFLAG this study N4865 mat A; his-3;
ash1-
3xFLAG::hph
Strain,
strain background
(N. crassa)		 ash1(Y888F) this study N4877 mat A; his-3;
ash1(Y888F)−
3xFLAG::hph;
∆mus-52::bar
Strain, strain
background
(N. crassa)		 ash1(Y888F) this study N4878 mat A; his-3;
ash1(Y888F)
−3xFLAG::hph
Strain, strain
background
(N. crassa)		 ash1(Y888F) this study N6268 mat A; his-3;
ash1(Y888F)
−3xFLAG::hph
Strain, strain
background
(N. crassa)		 ash1(Y888F) this study N6269 mat A; his-3;
ash1(Y888F)
−3xFLAG::hph
Strain, strain
background
(N. crassa)		 ash1(Y888F) this study N6875 mat a;
ash1(Y888F)−3x
FLAG::nat
Strain, strain
background
(N. crassa)		 ash1(Y888F) this study N6878 mat a;
ash1(Y888F)
−3xFLAG::nat;
∆mus-52::bar
Strain, strain
background
(N. crassa)		 ∆set-2 FGSC#15504 N5761 mat a;
∆set-2::hph
Strain, strain
background
(N. crassa)		 ∆set-2 this study N6335 mat A;
∆set-2::hph
Strain, strain
background
(N. crassa)		 set-2(∆SRI) this study N6956 mat A;
set-2(∆SRI)
::nat
Strain, strain
background
(N. crassa)		 ∆set-7 FGSC#
11182		 N4718 mat a;
∆set-7::hph
Strain, strain
background
(N. crassa)		 ∆set-7 Jamieson et al., 2018 N4730 mat A; ∆set-7::bar
Strain,
strain background
(N. crassa)		 ∆npf FGSC#
13915		 N4721 mat a; ∆npf::hph
Strain, strain
background
(N. crassa)		 ash1(Y888F);
∆set-2::nat 		this study N6266 mat A;
his-3; ash1(Y888F)
−3xFLAG::hph;
∆set-2::nat;
∆mus-52::bar
Strain, strain
background
(N. crassa)		 ash1(Y888F);
∆set-2 		this study N6267 mat A; his-3;
ash1(Y888F)
−3xFLAG::hph;
∆set-2::nat
Strain, strain
background
(N. crassa)		 ∆set-2;
∆set-7 		this study N6333 mat ?; ∆
set-2::hph;
∆set-7::bar
Strain, strain
background
(N. crassa)		 ∆set-2;
∆set-7 		this study N6334 mat ?;
∆set-2::hph;
∆set-7::bar
Strain, strain
background
(N. crassa)		 csr-1:
(TTAGGG)17		 Jamieson et al., 2018 N6383 mat a; csr-1:
(TTAGGG)17;
∆mus-52::bar
Antibody H3K36me3 Cell
Signaling 		Cat#4909S,
Clone (D5A7))		 immunoblot
(1:1000)
Antibody H3K36me2 Abcam Cat#ab9049 immunoblot
(1:1000)
Antibody Histone H3 Abcam Cat#ab1791 immunoblot
(1:2000)
Antibody IRDye
680RD
Goat-anti-Rabbit
secondary
antibody		 Licor Cat#926–68071 immunoblot
(1:5000)
Antibody H3K27me3 Millipore Cat#07–449 Chromatin
immuno
precipitation
Antibody H3K36me3 Abcam Cat#ab9050 Chromatin
immuno
precipitation
Antibody H3K36me2 Abcam Cat#ab9049 Chromatin
immuno
precipitation
Antibody H3K27ac ActiveMotif Cat#39133 Chromatin
immuno
precipitation
Antibody H3K27me2/3 ActiveMotif Cat#39535 Chromatin
immuno
precipitation