Key resources table.
| Reagent type (species) or resource |
Designation | Source | Identifier | Additional information |
|---|---|---|---|---|
| Chemical compound, drug |
Darpin D1 (Synthetic DNA) |
Invitrogen | N/A | |
| Chemical compound, drug |
GTP | Sigma | G-8877 | |
| Chemical compound, drug |
GDP | Sigma | G7127 | |
| Chemical compound, drug |
Crystallization plates |
TTP Labtech |
4150–05600 | |
| Chemical compound, drug |
Crystallization sparse matrix screens |
Qiagen | N/A | |
| Chemical compound, drug |
PEG-8000 | Sigma | 1546605 | |
| Chemical compound, drug |
PEG-2000 | Sigma | 8.21037 | |
| Chemical compound, drug |
Copper(II) sulfate |
Sigma | C1297 | |
| Chemical compound, drug |
1, 10- phenanthroline |
Sigma | 131377 | |
| Chemical compound, drug |
Trypsin | Sigma | T6567 | |
| Chemical compound, drug |
Chymotrypsin | Sigma | C6423 | |
| Chemical compound, drug |
Iodoacetamide | Sigma | I6125 | |
| Chemical compound, drug |
4-Vinylpyridine | Sigma | V3204 | |
| Other | 2:4:4 sk- Alp14-550:αβ- Tubulin:DRP |
Protein Data Bank |
PDB: #6MZF | Deposited Data (Atomic coordinates) |
| Other | 2:4:4 sk-Alp14-550- SL:αβ-Tubulin: DRP |
Protein Data Bank |
PDB: #6MZE | Deposited Data (Atomic coordinates) |
| Other | 1:2:1 sk-Alp14-550: αβ-Tubulin:DRPΔN |
Protein Data Bank |
PDB: #6MZG | Deposited Data (Atomic coordinates) |
| Other |
Saccharomyces
cerevisiae Stu2p |
UniprotKB/ Swiss-Prot |
P46675 | Protein sequence |
| Other |
Saccharomyces kluyveri
Stu2p or Alp14p |
Lachancea kluyveri NRRL Y-12651 chromosome |
SKLU-Cont10078 | Protein sequence |
| Other |
Schizosaccharomyces
pombe Alp14p |
UniprotKB/ Swiss-Prot |
O94534 | Protein sequence |
| Other |
Chaetomium
thermophilum Stu2 |
UniprotKB/ Swiss-Prot |
G0S3A7 | Protein sequence |
| Other | SoluBL21 bacterial expression system |
AmsBio | C700200 | Model system (expression system) |
| Recombinant DNA reagent |
pLIC_V2-Sc
Stu2p-H6 |
Current study |
N/A | Recombinant DNA constructs Expressed in bacterial strains |
| Recombinant DNA reagent |
pLIC_V2-Sk
Stu2p-H6 |
Current study | N/A | |
| Recombinant DNA reagent |
pLIC_V2-Sc Stu2-550-H6 (TOG1-TOG2 monomer) |
Al-Bassam et al., 2006 | ||
| Recombinant DNA reagent |
pLIC_V2-KL-Stu2 -monomer-H6 (residues 1–560) |
Current study | NA | |
| Recombinant DNA reagent |
pLIC_V2-CT Stu2-mon omer-H6 (residues 1–550) |
Current study |
NA | |
| Recombinant DNA reagent |
pLIC_V2-SK Alp14-monomer- H6 (residues 1-550) |
Current study | N/A | |
| Recombinant DNA reagent |
pLIC_V2-Sk Alp14-monomer -SL-H6 (residues 1–550; linker residues replaced KL sequence; see Materials and methods) |
Current study | N/A | |
| Recombinant DNA reagent |
pLIC_V2- sk-wt-Alp14- dimer-H6 (residues 1–724) |
Current study |
N/A | |
| Recombinant DNA reagent |
pLIC_V2 -sk-Alp14- dimer- H6 |
Current study |
N/A | |
| Recombinant DNA reagent |
S180C and L304C (residues 1–724) |
|||
| Recombinant DNA reagent |
pLIC_V2- sk-Alp14- dimer- H6 |
Current study | N/A | |
| Recombinant DNA reagent |
S41C and E518C (residues 1–724) |
|||
| Recombinant DNA reagent |
pLIC_V2-wt -Alp14-dimer- H6 (residues 1–690) |
Current study | N/A | |
| Recombinant DNA reagent |
pLIC_V2-TOG1M - H6 (residues 1–690: Y23A and R23A) |
Current study | N/A | |
| Recombinant DNA reagent |
pLIC_V2-TOG2M - H6 (residues 1–690: Y300A and K381A) |
Current study | N/A | |
| Recombinant DNA reagent |
pLIC_V2-INT1-H6
(residues 1–690: L206A, L208A, F275R D276A, L277A, V278A, K320L, R359A) |
Current study | N/A | |
| Recombinant DNA reagent |
pLIC_V2-INT2-H6
(residues 1–690: L39D, S40A, D42A, L437D, S440A, E478A and R479A) |
Current study | N/A | |
| Recombinant DNA reagent |
pLIC_V2 -INT1 + 2 H6 (L206A, L208A, F275R D276A, L277A, V278A, K320L, R359A L39D, S40A, D42A, L437D, S440A, E478A and R479A) |
Current study | N/A | |
| Recombinant DNA reagent |
pET303-H6-DRP | Current study |
N/A | |
| Recombinant DNA reagent |
pLIC_V2-H6-DRPΔN | Current study |
N/A | |
| Other | αβ-tubulin purified from porcine brains |
Current study |
N/A | Native protein purification |
| Other |
αβ-tubulin purified from porcine brains |
Castoldi and Popov, 2003 | ||
| Software, algorithm |
ASTRA V6.0 | Wyatt Technology |
http://www.wyatt.
com/products/ software/astra.html |
|
| Software, algorithm |
NanoAnalyze | TA Instruments |
http://www.tainstruments.com/ | |
| Software, algorithm |
EMAN2 | http://blake.bcm.edu/emanwiki/EMAN2 | ||
| Software, algorithm |
iMOSFLM | Battye et al., 2011 | http://www.mrc-lmb.cam.ac.uk/harry/imosflm/ver721/quickguide.html | |
| Software, algorithm |
PHASER | Terwilliger, 2000 | http://www.phaser.cimr.cam.ac.uk/index.php/ | |
| Software, algorithm |
PHASER | McCoy, 2007 | Phaser_Crystallographic_ Software |
|
| Software, algorithm |
PyMol | Schrodinger, LLC | http://www.pymol.org/ | |
| Software, algorithm |
UCSF-Chimera | Pettersen et al., 2004 | https://www.cgl.ucsf.edu/chimera/ | |
| Software, algorithm |
DM from CCP4 suite | Cowtan and Main, 1996 | http://www.ccp4.ac.uk/html/dmmulti.html | |
| Software, algorithm |
PHENIX | Adams et al., 2010 | https://www.phenix-online.org | |
| Software, algorithm |
anisotropy server | Strong et al., 2006 | https://services.mbi.ucla.edu/anisoscale/ | |
| Software, algorithm |
Phyre protein homology model |
Kelley et al., 2015 | www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index | |
| Software, algorithm |
Cr-yolo | Wagner et al., 2018 |
http://sphire.
mpg.de/wiki/ |
|
| Software, algorithm |
Relion 2.2 | Kimanius et al., 2016 | https://www2.mrc-lmb.cam.ac.uk/relion/index.php | |
| Software, algorithm |
Cryosparc | Punjani et al., 2017 | https://cryosparc.com/ | |
| Software, algorithm |
MolProbity | Chen et al., 2012 | http://molprobity.biochem.duke.edu | |
| Software, algorithm |
Coot | Emsley et al., 2010 | http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ | |
| Software, algorithm |
BLENDER 3D-animation |
Blender foundation |
https://www.blender.org/ |