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. 2017 Jun 8;26(16):3212–3220. doi: 10.1093/hmg/ddx204

Figure 4.

Figure 4.

LAMB4 protein levels in myenteric plexus of patients with LAMB4 variants. (A) 40X images of representative myenteric plexus in colonic tissue stained for S100, LAMB4 or DAPI from a non-diverticulitis control patient (1), a patient with sporadic diverticulitis but no damaging variants in LAMB4 (2), a patient with sporadic diverticulitis and heterozygous for the rs2074749 variant in LAMB4 (3) and the index patient (4). (B) Average LAMB4 expression in myenteric plexus in colonic tissue from Control, Sporadic and LAMB4 patients. LAMB4 expression was calculated for each patient sample as the average value of the total level of LAMB4 immunofluorescence in 13 separate myenteric plexus on average for each sample after subtracting the level of fluorescence in the surrounding tissue. Bars show the average of those values for four patients without diverticulitis (Control), eleven patients with diverticulitis but without a variant within the LAMB4 coding region (Sporadic) and six patients with diverticulitis carrying a variant within LAMB4 (LAMB4). Each bar represents the mean (± the standard error of the mean) of the average values from multiple plexus from the sampled tissues. (C) LAMB4 expression levels in colonic sections from individual patients who carried variants in LAMB4. LAMB4 expression was calculated for each patient sample as the average value of the total level of LAMB4 immunofluorescence in at least 13 separate myenteric plexus for each sample after subtracting the level of fluorescence in the surrounding tissue. Any value below zero was set to zero. Patient 1, index patient; patient 2, rs149874137; patient 3, chr7:107746309; patient 4, rs147992634; patient 5, rs2074749; patient 6, chr7:107720090.