Figure 3.

Degradation of the cholesterol rings in M. tuberculosis. Confirmed enzymes are denoted with the enzyme name(s) next to the reaction arrow. Pink text denotes the abbreviation of the catabolic intermediate chemical name. Black text indicates enzymes from M. tuberculosis that catalyze the specific reactions, and blue text indicates confirmed homologous enzymes from related Actinomyces spp. that catalyze the specific reactions. Asterisks indicate a tentative assignment, given that MOODA-CoA accumulates in a cholesterol-grown mutant of M. smegmatis that lacks FadE32 (Crowe et al.2017). The dashed arrow indicates a spontaneous, non-enzymatic, ring cleavage event in 9OHADD. Reactions indicated with red arrows indicate the release of acetyl-CoA, propionyl-CoA, pyruvate or succinyl-CoA. Brackets highlight stages in the pathway that require multiple enzymatic reactions.