Skip to main content
. 2016 Dec 30;26(4):702–716. doi: 10.1093/hmg/ddw431

Figure 5.

Figure 5

NDUFB10 is a substrate of CHCHD4. (A) NDUFB10 was detected in quantitative proteomics approaches as disulfide-linked interaction partner of CHCHD4. HEK293 cell lines stably expressing either CHCHD4-Strep wild type or the inactive C4S,C53S,C55S mutant were subjected to native and denaturing immunoprecipitation against the Strep tag. Subsequently, CHCHD4 interaction partners were identified by quantitative proteomics (data from (29)). (B) siRNA-mediated depletion of CHCHD4 results in decreased levels of NDUFB10. HeLa cells were transfected with siRNA directed against CHCHD4. 72 h after transfection cells were lysed and analyzed by immunoblot against NDUFB10. Depletion of CHCHD4 results in loss of NDUFB10.