Figure 2.

 Many miRNAs are DE between fetal and adult human erythroblast. Fetal and adult erythroblasts were differentiated ex vivo from CD34+ HSPCs collected from the fetal liver (FL) and adult bone marrow (BM). (A) PC analysis of the 500 most variable miRNAs. The first PC captures the developmental stage of the erythroblasts. (B) DE miRNAs that are up-regulated in fetal (red) and adult (blue) erythroblasts (FDR < 5%). The y-axis represents the log₂ of fetal-to-adult expression FC. The x-axis represents the mean normalized gene counts calculated by DESeq2. Most of the outstanding miRNA up-regulated in fetal erythroblasts are from the 14q32 locus. N.S., not significant. (C) Expression of the top 10 most up-regulated miRNAs in fetal erythroblasts, and of the 10 most up-regulated miRNAs in adult erythroblasts. Expression is reported in counts normalized using DESeq2’s regularized log₂ transformation. (D) Manhattan plot of DE miRNAs P-values (y-axis) based on the genomic location of their precursor sequences (x-axis).