(A-C) Immunostaining, real-time PCR and western blot analysis of vimentin and HMGB1 expression in donor and non-transplanted hearts (n=3/mice per group of three independent experiments, t-test; **P<0.01).
(D) Dectin-1 and TLR4 expression in graft infiltrating macrophages (n=3 mice/group of two independent experiments).
(E) Ly-6C expression in graft infiltrating macrophages from WT, dectin1 KO and TLR4 KO untreated recipient mice (n=3 mice/group of two independent experiments).
(F) Inflammatory cytokine production and chromatin immunoprecipitation of mouse monocytes trained with vimentin and HMGB1 (n=3 independent experiments, one-way ANOVA, **P<0.01; dashed line displays control non-trained conditions).
(G) Cytokine and lactate production of graft-infiltrating macrophages (n=4 mice/group of 2 independent experiments, one-way ANOVA, **P<0.01).
(H) Chromatin immunoprecipitation of graft-infiltrating macrophages (n=4 mice/group of 2 independent experiments, one-way ANOVA, *P<0.05; **P<0.01).