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. Author manuscript; available in PMC: 2019 Nov 20.
Published in final edited form as: Immunity. 2018 Nov 6;49(5):819–828.e6. doi: 10.1016/j.immuni.2018.09.008

Figure 1. Vimentin and HMGB1 are upregulated following organ transplantation and promote training of graft infiltrating macrophages.

Figure 1.

(A-C) Immunostaining, real-time PCR and western blot analysis of vimentin and HMGB1 expression in donor and non-transplanted hearts (n=3/mice per group of three independent experiments, t-test; **P<0.01).

(D) Dectin-1 and TLR4 expression in graft infiltrating macrophages (n=3 mice/group of two independent experiments).

(E) Ly-6C expression in graft infiltrating macrophages from WT, dectin1 KO and TLR4 KO untreated recipient mice (n=3 mice/group of two independent experiments).

(F) Inflammatory cytokine production and chromatin immunoprecipitation of mouse monocytes trained with vimentin and HMGB1 (n=3 independent experiments, one-way ANOVA, **P<0.01; dashed line displays control non-trained conditions).

(G) Cytokine and lactate production of graft-infiltrating macrophages (n=4 mice/group of 2 independent experiments, one-way ANOVA, **P<0.01).

(H) Chromatin immunoprecipitation of graft-infiltrating macrophages (n=4 mice/group of 2 independent experiments, one-way ANOVA, *P<0.05; **P<0.01).