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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: J Leukoc Biol. 2018 May 23;104(6):1049–1059. doi: 10.1002/JLB.3HI0118-015R

Figure 5.

Figure 5

Buprenorphine decreases CCL2-mediated chemotaxis of mature monocytes. Monocytes were untreated (Untx) or treated with buprenorphine (40 nM) (Bup) and immediately added to the top wells of a modified Boyden chamber. Chemotaxis was assayed to media or CCL2 (200 ng/mL) located at the bottom of the chamber. After 1h incubation at 37°C, cell migration was quantified by staining the cells that attached to the underside of the filter separating the top and bottom of the chamber. Data are expressed as mean fold change over base line chemotaxis, which was set to one as depicted by the dotted line, ± SD. n = 7 independent donors. &p<0.05 compared to baseline, *p<0.05 Top: Bup - Bottom: CCL2 compared to Top: untx - Bottom: CCL2.