Figure 5.

Restored Chemosensitivity in Mice with NRF2 Knockout in Tumors

(A) Experimental workflow of mouse xenograft. Athymic nude mice were subcutaneously injected with either wild-type A549 cells or NRF2-knockout A549 cells, and, once established tumors reached 100 mm³, they were treated with the first dose of chemotherapy on day 0. Mice were subsequently treated with chemo on days 3, 6, and 9. Tumor volumes were measured daily for 16 days until tumors reached 2,000 mm³. (B–D) Wild-type A549 or NRF2-knockout A549 tumors were treated with 2 mg/kg cisplatin (B), 5 mg/kg cisplatin with 5 mg/kg vinorelbine (C), 25 mg/kg carboplatin (D), or saline, and tumor size was measured for 16 days. Error bars represent ± SEM. Tumors (treated with 2 mg/kg cisplatin or saline) were extracted from both the wild-type A549 and NRF2-knockout A549 (2-11) implanted mice. (E) Representative tumors from each group are shown (n = 3).