Figure 1.

HEC-23 induces lysosomal enlargement in coelomocytes. (A) Representative images of endosomes and lysosomes in C. elegans coelomocytes. The top panel shows a schematic depiction of 3 pairs of coelomocytes (in red) in C. elegans. The bottom panels show a DIC image of a coelomocyte and images of 2xFYVE::GFP-labeled early endosomes, mCherry::CUP-5-labeled lysosomes, and LMP-1::GFP-labeled lysosomes. Scale bars, 10 μm. (B) Structures of HEC family compounds. (C and D) HEC family compounds induce enlargement of vacuoles in coelomocytes. Worms were treated with indicated HEC compounds at 100 μmol/L for 48 h. DIC images (C) are shown for the vacuoles and quantifications are shown in (D). (E and F) Representative DIC images (E) and quantification (F) of vacuole enlargement induced by HEC-23. (G) Effect of HEC-23 on vacuoles positive for 2xFYVE::GFP, mCherry::CUP-5, LMP-1::GFP and ASP-1::dsRed. Scale bars, 10 μm. (H and I) Quantification of vacuoles labeled with mCherry::CUP-5 (H) and LMP-1::GFP (I) in animals treated with HEC-23. (J) Quantification of lysosome sizes in worms treated with HEC-23 (100 μmol/L, 48 h). Data (mean ± SEM) were from 3 independent experiments. **P < 0.01, ***P < 0.001