Skip to main content
. 2018 Apr 2;9(12):1013–1026. doi: 10.1007/s13238-018-0520-0

Figure 3.

Figure 3

HEC-23 induces lysosomal damage in mammalian cells. (A and B) Images (A) and quantification (B) of HEC-23-induced enlargement of LAMP1-positive lysosomes in HeLa cells. (C) Quantification of HEC-23-induced lysosomal enlargement in the indicated cell types. (D) Representative images (left) and quantification (right) of EGFP-Gal3 in mCh-LAMP1-positive lysosomes in HeLa cells treated with HEC-23. (E) Representative images (left) and quantification (right) of LysoSensor Green staining in mCh-LAMP1-positive lysosomes in HeLa cells treated with HEC-23. (F) Representative images (left) and quantification (right) of cathepsin L-EGFP in mCh-LAMP1-positive lysosomes in HeLa cells treated with HEC-23. (G) Representative images (left) and quantification (right) of BODIPY-Pepstatin A in mCh-LAMP1-positive lysosomes in HeLa cells treated with HEC-23. In (D–G), boxed regions in the merged images are magnified and shown in the bottom right corners. Cells were treated with HEC-23 at 10 μmol/L for 3 h. Data (mean ± SEM) were from 3 independent experiments. **P < 0.01, ***P < 0.001. Bars represent 10 μm in all images