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. 2018 Apr 2;9(12):1013–1026. doi: 10.1007/s13238-018-0520-0

Figure 5.

Figure 5

HEC-23-induced enlargement of lysosomes depends on STAT3 activation. (A) Immuno-blotting of STAT3 phosphorylation at Y705 in HeLa cells following HEC-23 treatment. (B) Immuno-blotting of STAT3 in HeLa cells treated with siSTAT3. (C) Representative images (left) and quantification (right) of lysosomes labeled with mCherry-LAMP1 in HEC-23-treated HeLa cells pretreated with control siRNA (siCtrl) and STAT3 siRNA (siSTAT3). (D) qPCR-analysis of the expression of STAT3 target genes in siCtrl or siSTAT3 cells treated with HEC-23. (E) Representative images (left) and quantification (right) of lysosome damage in HEC-23-treated HeLa cells pretreated with control siRNA (siCtrl) and STAT3 siRNA (siSTAT3). The concentration of HEC-23 was 10 μmol/L in all treatments. Data representing mean ± SEM were derived from 3 independent experiments. **P < 0.01, ***P < 0.001 (Scale bars, 10 μm)