Figure 5.

Some confirmed hits reduced STAT phosphorylation in response to IFN-α2a treatment (150 IU/mL, 15 min) in HEK-Blue IFNα/β cells. (A) Representative western blots of 7 hits in three different concentrations. (B) and (C) Inhibition (%) of pSTAT1 and pSTAT3 after treatment. Protein levels were determined by western blotting and normalized to GAPDH. Two SDS gels were used for each experiment. Data represent means±SEM from at least three independent experiments. ^*P<0.05, ^**P<0.01, ^***P<0.001 compared to IFN-α2a treated cells.