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. 2018 Nov 23;8:17296. doi: 10.1038/s41598-018-35932-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis pipeline used throughout the study. The study was built through several steps: set up of mass cytometry analysis, process of samples on mass cytometer and data analysis. In this last step, several multidimensional data analysis tools were used, such as SPADE and viSNE, to highlight differences between septic shock patients and healthy donors in terms of cellular abundance and expression levels of exhaustion, activation and differentiation markers.