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. 2018 Nov 23;8:17296. doi: 10.1038/s41598-018-35932-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of phenotypic markers used for node characterisation. Median expression for each marker was averaged from all 10 samples for each of the 51 nodes. Results are shown on heatmap after hierarchical clustering. Colour intensity specifies the expression level. Combined expression of several markers allowed to identify characteristic leukocyte populations, but also rare cell subsets. For instance, two populations of CD3⁺ cells but double negative CD4⁻CD8⁻ were noted, both expressing TCRγδ, but showing different features, such as the expression level of CD8, CD16 and CD56.