Fig. 3.

Acetylation increases on proteins in immune response and metabolic pathways and decreases on transcriptional regulators. a Protein abundances in infection were normalized to mock abundances and plotted as log2 values. Four biological processes relevant to HCMV infection that display distinct protein abundance trends are illustrated. b K-means clustering was conducted in C3 (k = 7) to cluster changes in peptide acetylation levels at different time points of infection when compared with uninfected cells (right column). Whole-cell protein abundances (left) and peptide acetylation levels normalized to protein abundance (middle) are illustrated. Increased abundance, yellow; decreased abundance, blue; not detected in the proteome, gray. c–e Detected acetylation sites on proteins from indicated functional clusters representing site-specific regulation of acetylation during infection. Circles, proteins; squares, acetylation sites; red lines, previously unknown acetylation sites; black lines, known acetylation sites; red boxes, sites only detected during infection with abundances displayed on a 0–1 scale as they could not be normalized to mock samples; white bars, acetylated peptide not detected. c interferon response, d TCA cycle, e transcription regulation