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. 2018 Nov 23;8:17302. doi: 10.1038/s41598-018-35529-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunolabelling of methyl esterified and de-esterified pectin homogalacturonan in nematode infected Arabidopsis, aduki bean and maize root sections at 21 days post infection with Meloidogyne incognita. The LM20 antibody recognises methyl esterified pectic homogalacturonan and the LM19 antibody recognises de-esterified pectic homogalacturonan. Asterisks indicate giant cells in the nematode feeding site; N, nematode; Bars: 100 µm.