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. 2018 Nov 23;8:17312. doi: 10.1038/s41598-018-35531-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunoblot analysis on EVs and EAgo-1 immunoprecipitates. (A) EVs of both cell lines were subjected to immunoblot analysis for three common EV markers, which were clearly detected in the EV fractions purified from the extracellular media of both cell lines. (B) EAgo-1 IP was performed on the conditioned S2 and Cl.8 media as described in Methods section, but eluted in lithium dodecyl sulfate (LDS) instead of Qiazol. After elution, S2 and Cl.8 IP products were subjected to immunoblot analysis for EAgo-1, which was clearly detected in both IP products. Full-length blots are presented in Supplementary Fig. S1.