Table 2.
Small RNA sequencing data are in agreement with qRT-PCR results (Fig. 2).
| miRNA | S2 Cell | S2 EV | Log2FoldChange | padj value | ||||
|---|---|---|---|---|---|---|---|---|
| dme-bantam-3p | 9909 | 832 | 752 | 524 | 534 | 309 | −3.07 | 0.042 |
| dme-miR-2a-3p | 8 | 18 | 11 | 6 | 11 | 17 | −0.07 | 0.968 |
| dme-miR-2b-3p | 111 | 237 | 230 | 124 | 388 | 244 | 0.39 | 0.800 |
| dme-miR-2c-3p | 0 | 2 | 1 | 0 | 8 | 3 | 1.80 | 0.310 |
| dme-miR-14-3p | 1889 | 2519 | 1525 | 92 | 287 | 404 | −2.92 | 0.024 |
| dme-miR-34-5p | 610 | 728 | 481 | 255 | 468 | 228 | −0.94 | 0.434 |
| dme-miR-190-5p | 61 | 10 | 10 | 17 | 4 | 4 | −1.70 | 0.275 |
| dme-miR-276a-3p | 513 | 1165 | 942 | 392 | 1946 | 1375 | 0.50 | 0.736 |
| dme-miR-252-5p | 0 | 9 | 10 | 2 | 10 | 7 | 0.02 | 0.987 |
| dme-miR-1-3p | 1 | 1 | 2 | 8 | 0 | 4 | 1.50 | 0.377 |
| dme-miR-125-5p | NA | NA | NA | NA | NA | NA | NA | NA |
| dme-miR-210-3p | NA | NA | NA | NA | NA | NA | NA | NA |
| dme-let-7-5p | 4 | 0 | 1 | 6 | 27 | 13 | 3.10 | 0.052 |
| dme-miR-100-5p | 0 | 0 | 1 | 0 | 6 | 6 | 3.24 | 0.077 |
Small RNA sequencing data of S2 cells and derived EVs, on three biological replicates, were analysed using edgeR. Normalised counts per sample of the twelve miRNAs studied with qRT-PCR and their corresponding adjusted p-values (α < 0.05). Abundant miRNAs (low Ct values in qRT-PCR) display high read counts, while less abundant miRNAs (high Ct values in qRT-PCR) are characterized by (very) low read counts. NA values indicate miRNAs which have no reads in at least four out of six samples. Log2FoldChange values reflect the differential expression between S2 cells and EVs. Positive and negative values indicate upregulated and downregulated miRNA abundances in EVs, respectively. Abbreviations: EV: extracellular vesicle; padj: raw p-value adjustment.