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. 2018 Nov 23;8:17294. doi: 10.1038/s41598-018-35917-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental design to assess the effects of acute hyperglycemia on corneal tissue structure in vitro. Extension of nerve fibers are evident by 14 days post-construct assembly with growth continuing up to 42 days. Effects of high-glucose conditions were evaluated to determine if elevated environmental glucose levels (shown in blue) mediate changes in cell viability, phenotypic markers, and nerve morphology. Cell structures generated using Servier Medical Art licensed under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/).