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. 2018 Nov 23;8:17294. doi: 10.1038/s41598-018-35917-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of length (a) and density (b) of axons in day 28 and day 42 co-culture under high glucose concentrations for 14 days using 10X confocal images. Data was collected from n = 3 samples per condition and 5 regions per scaffold. Measurements and densities were determined using the ImageJ software plugin NeuronJ. Standard deviation is indicated as error bars for each group. *p < 0.05, **p < 0.005 and ***p < 5E-6, one-way ANOVA with a post-hoc Tukey test.