Fig. 1.

Top3β-TDRD3 stably associates with RISC in Drosophila. a Silver-stained SDS-PAGE analysis of the immunoprecipitated complexes of Top3β-TDRD3, FMRP, p68, and AGO2 from S2 cells; and a partial list of the number of peptides identified by MS analysis of each immunoprecipitate (bottom). The antibodies used for immunoprecipitation are indicated on the top, which include two against TDRD3 (A and C), one for FMRP, and HA antibodies for tagged p68-isoforms (RE and RC) and AGO2. Top3β, TDRD3, components of RISC, and FMRP-associated proteins are indicated by arrows on top and by brackets at the bottom. b IP-Western confirms co-IP of Top3β-TDRD3 with different components of RISC by two TDRD3 antibodies. c Reciprocal IP-Western shows that Top3β-TDRD3 co-IPs with HA-tagged p68, VIG, and AGO2 using transfected S2 cells and HA antibodies. Note that the level of TDRD3 in each IP is higher than that of Top3β, which is consistent with the MS data