Fig. 2.

TDRD3 acts as a scaffold linking Drosophila Top3β to RISC. a A cartoon shows similarity and differences between human and Drosophila Top3β-TDRD3 complexes (see Results for details). b Summary of domain-mapping experiment to show different mutants of TDRD3 (left), and their interactions with Top3β and components of RISC (right). The experiment was based on co-IP between different Flag-TDRD3 constructs and their interacting partners from transfected S2 cells. The detailed IP-Western data are in Supplemental figure (Supplementary Fig. 2A). The presence (+), absence (−), or reduction (down arrow), of interactions are indicated. c IP-Western shows that FLAG-Top3β co-IPs with reduced amount of FMRP and p68 in S2 cells depleted with TDRD3 by RNAi. A control experiment was included using RNAi that targets GFP. d IP-Western shows that FMRP co-IPs with reduced amount of Top3β, but normal amount of p68, in S2 cells inactivated of TDRD3 by CRISPR