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. 2017 Nov 28;6(4):126–135. doi: 10.1016/j.prnil.2017.11.001

Table 1.

Primer sequences used in this study RT-qPCR was performed with co-amplification of the genes with a reference gene by use of the cDNA template and corresponding gene-specific primer sets.

Genes and accession number Primers
BAX
NM_001291428.1
F: 5′-TTGCTTCAGGGTTTCATCCA-3′
R: 5′-CAGCCTTGAGCACCAGTTTG-3′
BID
NM_001244572.1
F: 5′- CCTACCCTAGAGACATGGAGAAG-3′
R: 5′- TTTCTGGCTAAGCTCCTCACG-3′
Bcl-2
NM_000633.2
F: 5′-ATGTGTGTGGAGAGCGTCAA-3′
R: 5′-ACAGTTCCACAAAGGCATCC-3′
Caspase 3
NM_001354779.1
F: 5′-GGCATTGAGACAGACAGTGG-3′
R: 5′-CATGGAATCTGTTTCTTTGC-3′
Caspase 8
XM_005246894.3
F: 5′-CTGCTGGGGATGGCCACTGTG-3′
R: 5′-TCGCCTCGAGGACATCGCTCTC-3′
Cytochrome c
NM_018947.5
F: 5′-AGTGGCTAGAGTGGTCATTCATTTACA-3′
R: 5′-TCATGATCTGAATTCTGGTGTATGAGA-3′
Caspase 9
NM_032996.3
F: 5′-GAGTCAGGCTCTTCCTTTG-3′
R: 5′-CCTCAAACTCTCAAGAGCAC-3′
Survivin
NM_001168.2
F: 5′-GACGACCCCATAGAGGAACA-3′
R: 5′-GACAGAAAGGAAAGCGCAAC-3′
Livin
NM_022161.3
F: 5′-TGGCCTCCTTCTATGACTGG-3′
R: 5′- ACCTCACCTTGTCCTGATGG-3′
p21Cip1
NM_001291549.1
F: 5′-GGCGTTTGGAGTGGTAGAAA-3′
R: 5′-GACTCTCAGGGTCGAAAACG-3′
p27Kip1
NM_004064.4
F: 5′-CCGGCTAACTCTGAGGACAC-3′
R: 5′-TTGCAGGTCGCTTCCTTATT-3′
p53
NM_001126118.1
F: 5′-GAGGTTGGCTCTGACTGTACC-3′
R: 5′-TCCGTCCCAGTAGATTACCAC-3′
Snail
NM_005985.3
F: 5′-AGACCCACTCAGATGTCAA-3′
R: 5′-CATAGTTAGTCACACCTCGT-3′
Twist
NM_000474.3
F: 5′- GGGAGTCCGCAGTCTTAC-3′
R: 5′- CCTGTCTCGCTTTCTCTTT-3′
c-Myc
NM_002467.5
F: 5′- AGCGACTCTGAGGAGGAACAAG-3′
R: 5′- CCTGCCTCTTTTCCACAGAAA-3′
GAPDH
NM_001289745.2
F: 5′-TTGGTATCGTGGAAGGACTCA-3′
R: 5′-TGTCATCATATTTGGCAGGTTT-3′