Figure 1.
Centrosome Amplification Induces Paracrine Invasion
(A) Experimental flowchart.
(B) Left, quantification of invasive structures. Right, normal and invasive 3D acini. White arrowheads indicate invasive protrusions. Scale bar: 20 μM.
(C) Quantification of centrosome amplification.
(D) Quantification of invasive structures.
(E) Quantification of invasive structures.
(F) Top, schematic representation of mammary organoids isolation and growth. Bottom, non-invasive and invasive mammary organoids. Scale bar: 20 μM.
(G) Quantification of invasive organoids.
(H) Images of zebrafish injected with cells with (+DOX) or without (−DOX) extra centrosomes (left) or co-injected +DOX/−DOX (right).
(I) Incidence of invasive cells in zebrafish embryos. Number of injected fish −DOX = 121; +DOX = 103; and co-injection +/−DOX = 116.
(J) Number of disseminated cells in the zebrafish tail. Error bars represent mean ± SEM.
For all graphics, error bars represent mean ± SD from three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns not significant.