Induction of Paracrine Invasion Is Mediated by RTK Signaling
(A) Left, schematic representation of the different CM treatments. Right, quantification of invasive structures.
(B) Quantification of invasive structures.
(C) Fold increase in RTK phosphorylation in MCF10A cells after incubation with CM+DOX.
(D) Left, quantification of invasive structures with or without HER2 (Trastuzumab, 40 μg/mL) and c-Met (PHA-66752, 1 μM) inhibitors. Right, acinar structures. Red arrowheads indicate invasive acini. Scale bar: 40 μM.
For all graphics, error bars represent mean ± SD from three independent experiments. ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns not significant.
See also Figure S2.