Figure 3.

Secretome Analysis Reveals Differential Protein Secretion in Cells with Amplified Centrosomes

(A) Experimental flowchart.

(B) Log₂-fold changes in protein abundance in the CM of cells with extra centrosomes (+DOX). Red circles indicate changes >1.5-fold difference.

(C) Pie charts represent the cellular localization of the proteins increased in CM+DOX. See STAR Methods for details.

(D) Fold change of secreted proteins in CM+DOX using protein array.

(E) IPA classification of the extracellular secreted proteins identified by mass spectrometry and protein arrays.

(F) Quantification of invasive structures after siRNA depletion.

(G) Left, validation of specific positive hits identified in (F). Right, acinar structures. Red arrowheads indicate invasive acini. For all graphics, error bars represent mean ± SD from three independent experiments. ^∗p < 0.05, ^∗∗p < 0.01. Scale bar: 40 μM.

See also Figure S3 and Table S1.