Figure 6.
Increased ROS Levels in Cells with Extra Centrosomes Drive Secretion
(A and B) (A) Levels of intracellular ROS using DCFDA or (B) by measuring the ratio of GSH/GSSG (48 hr).
(C) NRF2 protein levels in the cytosolic and nuclear fractions.
(D) Gene expression profile of cells with extra centrosomes (48 hr) compared to an NRF2 (NFE2L2)-induced gene-set signature.
(E) IL-8 secretion in after NAC treatment.
(F) Quantification of invasive structures.
(G) Left, cells stained for β-galactosidase (blue). Right, quantification of β-galactosidase positive cells. Scale bar: 40 μM.
(H) Left, quantification of γH2AX foci. Right, cells were stained for DNA (green) and γH2AX (magenta). L, large nuclei. Number of cells MCF10A.PLK4 −DOX = 469; −DOX+H2O2 = 518; +DOX = 466; +DOX+H2O2 = 377. Scale bar: 20 μM.
(I) Quantification of invasive structures.
(J) Ratio of GSH/GSSG.
(K) IL-8 secretion in cells treated with apocynin.
(L) Quantification of invasive structures. For all graphics error bars, represent mean ± SD from three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns not significant.