REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit Alexa-conjugated A488	Molecular Probes	#A11008; RRID: AB_143165
Mouse Alexa-conjugated A568	Molecular Probes	#A11011; RRID: AB_143157
Mouse Ki67 Alexa-conjugated A488	BD Biosciences	#561165; RRID: AB_10611866
Mouse Laminin V Alexa-conjugated A488	Millipore	#MAB19562X; RRID: AB_570380
Mouse α-tubulin	Sigma-Aldrich	#T9026; RRID: AB_477593
Rabbit centrin2 N-17-R	Santa Cruz	#sc-27793-R; RRID: AB_2082359
Mouse γ-H2AX S139	Merck Millipore	#05-636; RRID: AB_309864
Mouse α-smooth actin	Sigma-Aldrich	#A2547; RRID: AB_476701
Mouse p53	Santa Cruz	#sc-126; RRID: AB_628082
Rabbit p21	Cell Signaling	#2947-S; RRID: AB_823586
Rabbit β-actin	Cell Signaling	#4970; RRID: AB_2223172
Rabbit Histone H3	Cell Signaling	#9715S; RRID: AB_331563
Rabbit IL-8	Abcam	#ab106350; RRID: AB_10890102
Rabbit NRF2	Abcam	#ab62352; RRID: AB_944418
Rabbit ERK1/2	Cell Signaling	#4696; RRID: AB_390780
Rabbit p-ERK1/2 Thr202/Tyr204	Cell Signaling	#9101S; RRID: AB_331646
Rabbit MCAK	Bethyl Lab	#A300-807A-M; RRID: AB_577221
Mouse N-cadherin	BD Bioscience	#610920; RRID: AB_2077527
Mouse E-cadherin	BD Bioscience	#610181; RRID: AB_397580
Mouse Vimentin	BD Bioscience	#550513; RRID: AB_393716
Rabbit p-EGFR Tyr1068	Cell Signaling	#3777S; RRID: AB_2096270
Rabbit p-HER2 Tyr1221/1222	Cell Signaling	#2243S; RRID: AB_490899
Rabbit p-c-Met Tyr1234/1235	Cell Signaling	#3077S; RRID: AB_2143884
Chemicals, Peptides, and Recombinant Proteins
Doxycycline hyclate	Sigma-Aldrich	#D9891
RO-3306	Sigma-Aldrich	#SML0569
Trastuzumab	Genentech	N/A
Erlotinib	Santa Cruz	#sc-202154
PHA-66752	Sigma-Aldrich	#PZ0147
NSC23766	Millipore	#553502
Reparixin	Cayman Chemical	#21492
SCH563705	MedChem Express	#HY-10011
PD98058	Sigma-Aldrich	#P215
PP2	Sigma-Aldrich	#P0042
H2O2	Sigma-Aldrich	#H1009
N-acetyl cysteine	Sigma-Aldrich	#A9165
Mitotempo	Sigma-Aldrich	#SML0737
Nutlin-3	Sigma-Aldrich	#N6287
Doxorubicin	Sigma-Aldrich	#D1515
Apocynin	Santa Cruz	#sc-203321
Antimycin-A	Abcam	#ab141904
DMEM/F12	Sigma-Aldrich	#D8437
DMEM	Thermo Fisher Scientific	#41966-029
RPMI	Thermo Fisher Scientific	#21875-034
EGF	Sigma-Aldrich	#E4127
Insulin	Invitrogen	#12585-014
Hydrocortisone	Sigma-Aldrich	#H4001
Cholera toxin	Sigma-Aldrich	#C8052
Penicillin/Streptomycin	Thermo Fisher Scientific	#15140-122
Horse serum	Sigma-Aldrich	#H1138
FBS		#10500-064
Tet-free FBS	Hyclone	#SH30070.03T
Blasticidin	Generon	#2805-10
Geneticin (G418)	Thermo Fisher Scientific	#10131027
Polybrene	Sigma-Aldrich	#H9268
Immobilized trypsin, TPCK treated agarose resin	Thermo Fisher Scientific	#20230
CellTracker Green CMFDA	Thermo Fisher Scientific	#C2925
CellTracker CM-Dil Dye Red	Thermo Fisher Scientific	#C7001
Tricaine	Sigma-Aldrich	#E10521
Formaldehyde 16%	Thermo Fisher Scientific	#28908
Formalin	Sigma-Aldrich	#HT5012
Phalloidin Alexa A568	Molecular Probes	#12380
Hoechst 33342	Molecular Probes	#H3570
Mitosox	Molecular Probes	#M36008
ProLong anti-fade mounting medium	Molecular Probes	#P36934
BSA	Sigma-Aldrich	#A9647
Lipofectamine 2000	Invitrogen	#11668027
Lipofectamine RNAi Max	Invitrogen	#13778075
Power SYBR Green PCR Master Mix	Applied Biosystems	#4367659
RIPA Buffer	Thermo Scientific	#89901
Complete Mini Protease Inhibitor Cocktail	Roche	#11836153001
Phosphatase inhibitor Cocktail	Cell Signaling	#5870
Bradford Protein Assay	Bio-Rad	#5000006
Insulin-Transferrin-Selenium-Ethanolamine (ITS)	Thermo Fisher Scientific	#51500-056
Liberase Research Grade	Sigma-Aldrich	5401020001
Human IL-8	R&D	#208-IL-010
Human C-terminal ANGPTL4	R&D	#4487-AN-050
Human GDF-15	Invitrogen	#EHGDF-15
Human FGF	Sigma-Aldrich	#F0291
Critical Commercial Assays
ELISA GDF-15 kit	Thermo Fisher Scientific	#EHGDF15
ELISA IL-8 kit	Abcam	#ab46032
ELISA PAI kit	Abcam	#ab108891
ELISA Mesothelin kit	R&D	#DMSLN0
ELISA Angiopoietin-like 4 kit	Thermo Fisher Scientific	#EHANGPTL4
ELISA HMGB1 kit	IBL	#ST51011
RNAeasy kit	Qiagen	#74104
High-capacity RNA-to-cDNA kit	Thermo Fisher Scientific	#4387406
Power SYBR Green	Thermo Fisher Scientific	#4367659
Pierce LDH Cytotoxicity Assay kit	Thermo Fisher Scientific	#88953
Senescent cells histochemical staining kit	Sigma-Aldrich	#CS0030-1KT
Cellular Reactive Oxygen Species detection kit	Abcam	#Ab113851
GSH/GSSH-Glo assay	Promega	#V6611
BCA Protein assay	Thermo Fisher Scientific	#23225
Human phosphor-receptor tyrosine kinase array kit	R&D	#ARY001B
Proteome Profiler Human XL Oncology array kit	R&D	#ARY026
Custom Human antibody array kit	RayBiotech	#AAH-CUST-M
Deposited Data
Microarray Data	ArrayExpress	E-MTAB-6415
Experimental Models: Cell Lines
MCF10A	ATCC	#CRL-10317
MCF10A.PLK4	Godinho et al. (2014)	N/A
HaCaT.PLK4	Godinho et al. (2014)	N/A
BF.PLK4	This work	N/A
RPE1.PLK4	Rhys et al. (2018)	N/A
MCF-7.PLK4	This work	N/A
HCC1954.PLK4	This work	N/A
BT-549	Prof. Peter Schmid (QMUL)	N/A
MDA-MB-231	Prof. Peter Schmid (QMUL)	N/A
MDA-MB-468	Prof. Peter Schmid (QMUL)	N/A
HEK293M	Prof. David Pellman (DFCI)	N/A
Experimental Models: Organisms/Strains
Mouse: Mus musculus C57BL/57 strain	Charles River	JAX C57BL/6J
Mouse: Mus musculus B6.129S2(C) – Cxcr2tm1Mwm/J	Jackson Laboratory	#006848
Zebrafish: Danio rerio mitfaw2/w2; mpv17a9/a9 (Casper)	N/A	N/A
Oligonucleotides
siRNAs – See Table S6		N/A
qRT-PCR primers – See Table S7		N/A
Recombinant DNA
pLenti-CMV-TetR-Blast	Addgene	#17492
pLenti-CMV/TO-Neo-DEST.PLK4	Godinho et al. (2014)	N/A
pInducer.PLK4	Rhys et al. (2018)	N/A
pMD2.G VSV-G	Addgene	#12259
psPAX2 Gag-Pol	Addgene	#12260
Other
Vivaspin columns MWCO 5000 Da	GE Healthcare	#28-9323-59
8-well chamber slides	Corning	#354108
8-well chamber slides with glass bottom	ibidi	#80827