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Western blotting assay for MKN45 cells. MKN45 cells were transfected with HER-3 siRNAs and harvested 72 hr after transfection. The cells were lysed and 50 mg of total protein was used for detection of ErbB3 by western blotting. HER-3 protein level was decreased after treatment with siRNA. Beta-actin was used as internal control of western blot.
