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Studying FIX activity in stable S2-FIX cells and transfected Drosophila S2 cells by TGE technique. Following induction of transient or stable S2 cells with 0.5 mM CuSO4 in the presence of 6 μg/ml vitamin K1, at various post induction times, the hFIX coagulation activity of the cultured media was examined by performing clotting test, using immunodepleted plasma for the hFIX and aPTT reagent. The data are the means±S.D. of 3 similar experiments.
