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Density gradient subcellular fractionation results in highly pure rat brain cortical mitochondria. Enrichment of complex II (succinate dehydrogenase [SDH]) activity was spectrophotometrically measured for cortical mitochondria isolated using discontinuous sucrose gradient ultracentrifugation method. SDH activity levels were enriched by 6.5 ± 0.7 (mean ± SD) times compared to homogenate. Data is presented as mean ± standard error of the mean [SEM] (n = 4).
