FIGURE 6.

The expression level of runt-related transcription factor 2 (Runx2) in mesenchymal stem cells (MSCs) on different days of transfection. Fold increase ± standard error of the mean (SEM) of Runx2 expression was compared between MSCs transfected with plenti-III-mir-green fluorescent protein (GFP) plasmid bearing pre-miR-210, MSCs transfected with Scramble and MSCs transfected with pmaxGFP vector on days 0, 7, 14 and 21. The highest expression of Runx2 was detected on day 7. The difference in gene expression was calculated using the 2-ΔΔCT method (p < 0.05). The difference in Runx2 gene expression was statistically significant between miR-210-bearing plasmid and two control groups on day 7 (p < 0.01) and 14 (p < 0.027), but not on day 21.