FIGURE 7.

The expression level of alkaline phosphatase (ALP, ALPL) gene in mesenchymal stem cells (MSCs) on different days of transfection. Fold increase ± standard error of the mean (SEM) of ALP expression was compared between MSCs transfected with plenti-III-mir-green fluorescent protein (GFP) plasmid bearing pre-miR-210, MSCs transfected with Scramble and MSCs transfected with pmaxGFP vector on days 0, 7, 14 and 21. The highest ALP gene expression was detected on day 21. The difference in gene expression was calculated using the 2-ΔΔCT method (p < 0.05). The difference in ALP gene expression was statistically significant between miR-210-bearing plasmid and two control groups at all time points (p < 0.026 at day 7, p < 0.02 at day 14, p < 0.017 at day 21).