FIGURE 8.

The expression level of osteocalcin or bone gamma-carboxyglutamic acid-containing protein (BGLAP) gene in mesenchymal stem cells (MSCs) on different days of transfection. Fold increase ± standard error of the mean (SEM) of osteocalcin gene expression was compared between MSCs transfected with plenti-III-mir-green fluorescent protein (GFP) plasmid bearing pre-miR-210, MSCs transfected with Scramble and MSCs transfected with pmaxGFP vector on days 0, 7, 14 and 21. The highest expression of osteocalcin gene was detected on day 14. The difference in gene expression was calculated using the 2-ΔΔCT method (p < 0.05). The difference in osteocalcin gene expression was statistically significant between miR-210-bearing plasmid and two control groups at all time points (p < 0.017 at day 7, p < 0.015 at day 14, p < 0.019 at day 21).