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Serum inhibits sphere-induced EMT. Colony-forming assay from single cells issued from 10-day (sph1) or 30-day (sph3) CAL33 sphere cultures in EFI medium in the absence (left) or presence (right) of 10% serum. CAL33 cells: Human tongue squamous cell carcinoma line; EMT: Epithelial-mesenchymal transition; EFI: Serum-free medium with EGF, FGF and insulin; EGF: Epidermal growth factor; FGF: Fibroblast growth factor; FBS: Fetal bovine serum.
