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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Fungal Genet Biol. 2018 Sep 25;121:56–64. doi: 10.1016/j.fgb.2018.09.008

Fig 4.

Fig 4.

The Bwc1C605A and Bwc1C605S isoforms physically interact with Bwc2 in yeast two hybrid assays. The cDNAs of either gene were cloned adjacent to the activation (AD) or DNA binding (BD) domains of S. cerevisiae Gal4. Constructs were transformed into an S. cerevisiae reporter strain. The β-galactosidase activity and growth on medium without adenine (ade) indicate interactions between Bwc2 and the Bwc1 alleles to reconstitute the Gal4 protein. There was no statistical difference between the β-galactosidase activities of the wild type vs. mutant isoforms.