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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Brain Struct Funct. 2018 Sep 8;223(9):4227–4241. doi: 10.1007/s00429-018-1748-4

Figure 5.

Figure 5.

ROCK1 or ROCK2 heterozygosity reduces pLIMK in synaptosome fractions. N=5-10 mice per genotype. Representative Western blots from whole homogenate (WH) and synaptosome (Syn) fractions of (A-B) ROCK1+/+ and ROCK1+/− or (D-E) ROCK2+/+ and ROCK2+/− mice. For densitometry analysis protein levels of WH or Syn homogenates from ROCK1+/− or ROCK2+/− were normalized to ROCK1+/+ or ROCK2+/+, respectively. WH and Syn protein levels measured by densitometry were averaged to generate mean per genotype. C ROCK1+/− WH showed decreased ROCK1 (t-test: **P=0.0024, t=4.369, df=8), elevated pLIMK levels (t-test: *P=0.0355, t=2.429, df=10), and increased pCofilin levels (t-test: *P=0.0315, t=2.499, df=10). ROCK1+/− Syn fractions showed decreased ROCK1 (t-test: ***P=0.0003, t=5.099, df=11), decreased pLIMK (t-test: *P=0.0265, t=2.600, df=10), reduced pCofilin levels (t-test: ***P=0.0008, t=4.580, df= 11), and decreased pMLC (t-test: *P=0.0130, t=3.089, df=9). F ROCK2+/− WH showed decreased ROCK2 (t-test: ****P<0.0001, t=7.273, df=11), elevated pLIMK (t-test: ***P=0.0004, t=5.000, df=11), and increased pCofilin levels (t-test: *P=0.0136, t=2.988, df=10). ROCK2+/− Syn fractions showed decreased ROCK2 (t-test: **P=0.0068, t=3.261, df=12), decreased pLIMK (t-test: *P=0.0253, t=2.554, df=12), and reduced pCofilin levels (t-test: ***P=0.0067, t=3.334 df=11). Bar graphs plot the mean with standard error of the mean. WH, whole homogenate; Syn, synaptosome fractions; R1+/+, ROCK1+/+ ; R1+/−, ROCK1+/− ; R2+/+, ROCK2+/+ ; R2+/−, ROCK2+/−.