Skip to main content
. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Brain Struct Funct. 2018 Aug 14;223(9):3945–3958. doi: 10.1007/s00429-018-1731-0

Figure 1. Identification of claustrum using fluorescently-labeled anterior cingulate cortex (ACC) afferents.

Figure 1.

(A) Photomicrograph showing injection of adeno-associated virus expressing channelrhodopsin-2 and mCherry tag (purple, pseudo-colored) in ACC. The dotted lines outline the corpus callosum (CC). (B) Photomicrograph showing ACC afferents expressing mCherry (purple) in the contralateral claustrum (CL) and neurobiotin-filled spiny claustrum neurons (green) targeted for whole-cell electrophysiology. The neighboring cortex (CTX) and striatum (STR) are indicated and devoid of labeled fibers. (C) Inset from (B) showing the filled spiny claustrum neurons at higher magnification. (D-E) Photomicrograph showing a dendritic process from a spiny (D) or aspiny (E) claustrum neuron. Scale bars = 400 μm (B), 100 μm (C), 10 μm (D, E).