Table 2.
Cellular localization of complement components in adulthood and aging.
| Cell type | Localization | Experimental model | Method | Ref. |
|---|---|---|---|---|
| C1q | ||||
| Neuron | Synaptic puncta |
In vivo: C57BL/6 mice Human tissue |
IHC | [32] |
| Microglia | Cellular |
In vitro: mice primary culture In vivo: C57BL/6, C1qa deletion mice |
qRT-PCR, IF, IHC, WB | [30–33] |
| Astrocyte | Exosomes | Human plasma | Immunoassay | [34] |
| C3 | ||||
| Microglia | Cellular | In vitro: mice primary cultures | qRT-PCR, IF, ICC, WB | [30, 31, 35] |
| Astrocyte | Cellular | In vitro: mice primary cultures | qPCR, IF, WB, RNA-seq | [30, 35, 36] |
| Exosomes | Human plasma | Immunoassay | [34] | |
| CR3 | ||||
| Microglia | — | In vitro: mice primary culture | qRT-PCR, IF | [31] |
| C3aR | ||||
| Neuron | Cellular |
In vitro: cultured neural stem cells, rat In vivo: WT mice and rat |
In situ hybridization, IF | [41, 42] |
| Microglia | Cellular |
In vivo: WT mice Human tissue |
In situ hybridization, IF, IMC | [37–39] |
| Astrocyte | Cellular | In vitro: CB193 cell line, human astrocyte cultures | IF, RT-PCR, IP | [39, 40] |
qRT-PCR: semiquantitative PCR; ICC: immunocytochemistry; IHC: immunohistochemistry; WB: Western blot; IF: immunofluorescence; FACS: fluorescence-activated cell sorting; IP: immunoprecipitation.