Table 1.

Stimulation of NHEJ repair pathway by a terminal ribonucleotide

Substrate	Terminal C	Relative Joining efficiency#	% Direct ligation	Ligation Stimulation+
--GC  [--	deoxyC	1	87 ± 6	1
--]  CG--	(ribo)C	1.2 ± 0.7	92 ± 1	1.3
	deoxyC	1	19 ± 8	1
--GAC  [--	(ribo)C	5.8 ± 1.9	88 ± 6	26
--]      AG--	2’fluoroC	5.4 ± 3.9	88 ± 1	25
	arabinoC	1.1 ± 0.6	< 2*	< 0.1*
--GACGC  [--	deoxyC	1	32 ± 3	1
--]      GCAG--	(ribo)C	6.3 ± 3.1	88 ± 3	17
--TTTTTTTTGC  [--	deoxyC	1	4 ± 1	1
--]                              G--	(ribo)C	3.0 ± 0.9	64 ± 5	48

^#Joining efficiency rC/joining efficiency dC, as measured by qPCR that amplifies all NHEJ products.

^*Direct ligation product was undetectable

⁺Ligation stimulation=Relative joining efficiency multiplied by %Direct ligation rC/%Direct ligation dC